Rucka, who is writing the reborn comic series, said that as Wonder Woman comes from the fictional all-female island nation of Themyscira “the only options are women”.
However, he did not rule out the potential for the character to have a romantic relationship with Steve Trevor, Wonder Woman’s love interest in previous incarnations of the character.
Rucka told the Comicosity website: “There was a piece of me that was taken aback when Wonder Woman #2 came out and I saw that some people thought (Wonder Woman’s sexuality) was still unclear.
“I don’t know how much clearer I can make it!”
The US writer said the character’s sexuality was secondary to the narrative of the comic book series.
Rucka said: “It doesn’t matter if I say ‘Yes, she’s queer’ or ‘No, she’s not queer’
“It matters what you get out of the book.
“Can you find it? Is it there? Is it on the page in action or in deed? Then, there’s your answer.”
The writer criticised other comic books for the way in which characters’ sexualities have been disclosed.
He said: “The character has to stand up and say ‘I’M GAY’ in all bold caps for it to be evident.
“For my purposes, that’s bad writing. That’s a character stating something that’s not impacting the story.”
Rucka, who has previously written Batman and Superman comics, added that DC Comics would “prefer this not to be an issue to anybody”.
He said: “Nobody at DC has ever said ‘She’s gotta be straight’.
“Nobody ever. They’ve never blinked at this.”
Wonder Woman was created by US psychologist and writer William Moulton Marston and first appeared in a comic book in 1941.
One of DC Comics’ best-known characters, Wonder Woman appeared on the big screen this year in Batman v Superman: Dawn of Justice.
A standalone film is set to hit cinemas in June 2017, starring Gal Gadot.